LOCUS       EXTL1-pGEn2             7245 bp    DNA     circular     20-SEP-2016
FEATURES             Location/Qualifiers
     CDS             129..1108
                     /dnas_title="AmpR"
                     /label=AmpR
                     /vntifkey="4"
     rep_origin      1137..1804
                     /dnas_title="Col E1 origin"
                     /label=Col E1 origin
                     /vntifkey="33"
     misc_feature    1998..2127
                     /note="5'ITR"
     misc_feature    2208..2790
                     /note="CMV Promoter"
     misc_feature    2828..2960
                     /note="Artificial Intron"
     misc_recomb     complement(3839..3867)
                     /label=attB1
                     /note="attB1 site"
                     /note="recognized as attB1"
     misc_feature    5873..6414
                     /note="WPRE"
     misc_recomb     5835..5857
                     /label=attB2
                     /note="attB2 site"
                     /note="recognized as attB2"
     misc_feature    complement(6421..6635)
                     /note="BGH pA"
     misc_feature    complement(6723..6852)
                     /note="3'-ITR"
     misc_feature    2982..3056
                     /note="TCM signal sequence"
     misc_feature    3060..3083
                     /note="His tag"
     misc_feature    3084..3134
                     /note="AviTag"
     misc_feature    3135..3830
                     /note="Superfolder GFP"
     misc_feature    2976..2981
                     /note="Kozak seq"
     misc_feature    3868..3890
                     /note="TEV site"
     misc_feature    3891..5828
                     /note="OPTIMIZED EXTL1 coding region"
     vector          join(5843..7245,1..3857)
                     /source="pGEn2-DEST"
                     /type="Destination Vector"
     clone           3858..5842
                     /source="EXTL1 PDONR221   "
                     /type="Entry Clone"
     source          1..7245
                     /dnas_title="EXTL1-pGEn2"
ORIGIN      
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA
     1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG
     1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG
     2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG
     2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT
     2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG
     2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
     2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
     2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
     2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
     2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
     2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG
     2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC
     2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA
     2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
     2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC
     2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT
     2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA
     2941 CTTTGCCTTT CTCTCCACAG GTGTCCAgcg gccgcGCCAC cATGAGACTG CTGACCGCCC
     3001 TGTTCGCCTA CTTCATCGTG GCCCTGATCC TGGCCTTCAG CGTGTCCGCC AAGAGCATGC
     3061 ACCACCACCA TCACCATCAT CACATGAGCG GCCTGAACGA CATCTTCGAG GCCCAGAAAA
     3121 TCGAGTGGCA CGAGATGAGC AAGGGCGAGG AACTGTTCAC CGGCGTGGTG CCCATCCTGG
     3181 TGGAACTGGA CGGCGACGTG AACGGCCACA AGTTCTCTGT GCGGGGCGAG GGCGAGGGGG
     3241 ACGCCACAAA TGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGAAAG CTGCCCGTGC
     3301 CCTGGCCTAC CCTGGTCACA ACCCTGACCT ACGGCGTGCA GTGCTTCAGC AGATACCCCG
     3361 ACCACATGAA GCGGCACGAT TTCTTCAAGA GCGCCATGCC CGAGGGCTAC GTGCAAGAAC
     3421 GGACCATCAG CTTCAAGGAC GACGGCACCT ACAAGACCAG AGCCGAAGTG AAGTTCGAGG
     3481 GCGACACCCT GGTCAACCGG ATCGAGCTGA AGGGCATCGA CTTCAAAGAG GACGGCAACA
     3541 TCCTGGGCCA CAAGCTCGAG TACAACTTCA ACAGCCACAA CGTGTACATC ACCGCCGACA
     3601 AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGGCA CAACGTGGAA GATGGCAGCG
     3661 TGCAGCTGGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC
     3721 CCGACAATCA CTACCTGAGC ACCCAGAGCG TGCTGAGCAA GGACCCCAAC GAGAAGCGGG
     3781 ACCACATGGT GCTGCTGGAA TTTGTGACCG CCGCTGGCAT CACCCACGGC GAATTCgcAT
     3841 CAACAAGTTT GTACAAAAAA GCAGGCTctg aaaacttgta ctttcaaggc AGACTGGCCC
     3901 TGCCTCCTAG ACCTAGACCT GGTGCTTCTC AAGGCTGGCC TAGATGGCTG GATGCCGAGC
     3961 TGCTGCAGAG TTTTTCTCAG CCTGGCGAGC TGCCTGAGGA TGCCGTTTCT CCACCTCAAG
     4021 CTCCTCACGG CGGCAGCTGT AATTGGGAGA GCTGCTTCGA CACCAGCAAG TGTAGAGGCG
     4081 ACGGCCTGAA GGTGTTCGTG TATCCTGCCG TGGGCACCAT CAGCGAGACA CACAGAAGAA
     4141 TCCTGGCCAG CATCGAGGGC AGCAGATTCT ACACATTTTC CCCAGCCGGC GCTTGCCTGC
     4201 TGCTGCTTCT TTCTCTTGAT GCCCAAACCG GCGAGTGCAG CTCTATGCCC CTGCAATGGA
     4261 ACAGAGGCCG GAACCATCTG GTGCTGAGAC TGCATCCTGC TCCATGTCCT AGAACCTTCC
     4321 AGCTCGGACA GGCCATGGTG GCCGAAGCTT CTCCAACCGT GGATAGCTTC AGACCCGGCT
     4381 TCGATGTGGC TCTGCCTTTC CTGCCTGAAG CTCATCCTCT TAGAGGCGGA GCACCTGGAC
     4441 AGCTGAGACA GCATTCTCCT CAACCTGGCG TGGCACTGCT GGCCCTTGAA GAAGAAAGAG
     4501 GCGGCTGGCG CACAGCTGAT ACAGGATCTT CTGCCTGTCC TTGGGACGGC AGATGCGAAC
     4561 AAGATCCTGG ACCTGGCCAG ACACAGAGAC AAGAAACCCT GCCTAACGCC ACCTTCTGTC
     4621 TGATCTCTGG ACACAGACCT GAGGCCGCCT CTAGATTTCT GCAAGCCCTG CAGGCCGGAT
     4681 GTATCCCTGT TCTGCTGTCT CCTAGATGGG AGCTGCCCTT CAGCGAAGTG ATCGATTGGA
     4741 CAAAGGCCGC CATCGTGGCT GACGAGAGAC TGCCTCTTCA AGTGCTGGCC GCTCTGCAAG
     4801 AGATGTCTCC TGCTAGAGTG CTGGCTCTGA GGCAGCAGAC CCAGTTTCTG TGGGATGCCT
     4861 ACTTCAGCAG CGTGGAAAAA GTGATCCACA CCACACTGGA AGTGATTCAG GACCGGATCT
     4921 TCGGCACAAG CGCCCATCCA AGCCTGCTGT GGAATTCTCC TCCTGGTGCT CTGCTGGCAC
     4981 TGAGCACCTT TTCTACTAGC CCTCAGGACT TCCCATTCTA CTACCTGCAG CAGGGCTCCA
     5041 GACCAGAGGG CAGATTTTCT GCCCTGATCT GGGTTGGACC ACCAGGCCAA CCTCCACTGA
     5101 AACTGATTCA AGCCGTGGCC GGATCTCAGC ACTGTGCCCA GATTCTGGTC CTGTGGTCCA
     5161 ATGAGAGGCC CCTGCCTAGC AGATGGCCTG AAACAGCTGT GCCCCTGACC GTGATCGACG
     5221 GACACAGAAA GGTGTCCGAC CGGTTCTACC CCTACTCCAC CATCAGAACC GATGCCATCC
     5281 TGAGCCTGGA TGCCAGAAGC AGCCTGAGCA CATCTGAGGT GGACTTCGCC TTTCTCGTGT
     5341 GGCAGAGCTT CCCCGAGAGA ATGGTCGGAT TTCTGACCAG CAGCCACTTC TGGGATGAAG
     5401 CCCATGGCGG ATGGGGATAC ACCGCCGAGA GAACAAACGA GTTCAGCATG GTGCTGACCA
     5461 CCGCCGCCTT CTACCACCGG TACTACCACA CACTGTTCAC CCACAGCCTG CCTAAGGCAC
     5521 TGAGAACACT GGCCGATGAG GCCCCTACAT GTGTGGACGT GCTGATGAAT TTCATCGTGG
     5581 CCGCTGTGAC CAAGCTGCCT CCAATCAAGG TGCCATACGG CAAGCAGAGG CAAGAAGCTG
     5641 CTCCTCTTGC TCCTGGTGGA CCCGGACCAA GACCTAAACC TCCTGCTCCT GCACCTGACT
     5701 GCATCAATCA GATCGCCGCT GCCTTTGGCC ACATGCCACT GCTGAGTAGC AGACTGAGAC
     5761 TGGACCCCGT GCTGTTCAAG GATCCAGTGT CTGTGCAGCG GAAGAAGTAC AGAAGCCTGG
     5821 AAAAGCCTTA GGACCCAGCT TTCTTGTACA AAGTGGTTGA TAGCTTGGAT CCAATCAACC
     5881 TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC
     5941 GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT
     6001 CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT
     6061 TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG
     6121 CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC
     6181 GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC
     6241 TGACAATTCC GTGGTGTTGT CGGGGAAGCT GACGTCCTTT CCATGGCTGC TCGCCTGTGT
     6301 TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC
     6361 GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGAGATCT
     6421 GCCTCGACTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC
     6481 TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG
     6541 CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG
     6601 GAGGATTGGG AAGACAATAG CAGGCATGCT GGGGACTCGA GTTAAGGGCG AATTGCCGAT
     6661 TAGGATCTTC CTAGAGCATG GCTACGTAGA TAAGTAGCAT GGCGGGTTAA TCATTAACTA
     6721 CAAGGAACCC CTAGTGATGG AGTTGGCCAC TCCCTCTCTG CGCGCTCGCT CGCTCACTGA
     6781 GGCCGGGCGA CCAAAGGTCG CCCGACGCCC GGGCTTTGCC CGGGCGGCCT CAGTGAGCGA
     6841 GCGAGCGCGC AGCCTTAATT AAGAGCTCAT GGCGCGCCTA GGCCTTGACG GCCTTCCGCC
     6901 AATTCGCCCT ATAGTGAGTC GTATTACGTC GCGCTCACTG GCCGTCGTTT TACAACGTCG
     6961 TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC
     7021 CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGAAACGCCC TTCCCAACAG TTGCGCAGCC
     7081 TGAATGGCGA ATGGGAGCGC CCTGTAGCGG CCACTCAACC CTATCTCGGT CTATTCTTTT
     7141 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA
     7201 AAATTTAACG CGAATTTTAA CAAAATATTA ACGCTTACAA TTTAG
//

